thr77 Secrets
thr77 Secrets
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in many tumors, which results in chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two supplemental splice variants have been reported but specific nucleotide sequence details is not still accessible.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This information outlines even further achievable optimization of cellular and immunoassay parameters to make certain the absolute best success are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to guarantee the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope over the concentrate on protein. By evaluating the staining through the blocked antibody vs . the antibody by yourself, you can see which staining is certain.
Concentrate on Information Cyclin E belongs into the really conserved cyclin spouse and children, whose customers are characterized by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins show unique expression and degradation check here patterns which contribute on the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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